
2022
38. Patel A and Peralta-Yahya P. Olfactory receptors as an emerging chemical sensing scaffold, Biochemistry, 62, 187-195, 2022.
In this Perspective, we briefly overview recent advances in the biosensing of small molecules, including nucleic acid aptamers, allosteric transcription factors, and two-component systems. We then look more closely at a recently developed chemical sensing system, G protein-coupled receptor (GPCR)-based sensors. Finally, we consider the chemical sensing capabilities of the largest GPCR subfamily, olfactory receptors (ORs). We examine ORs’ role in nature, their potential as a biomedical target, and their ability to detect compounds not amenable for detection using other biological scaffolds. We conclude by evaluating the current challenges, opportunities, and future applications of GPCR- and OR-based sensors.
38. Patel A and Peralta-Yahya P. Olfactory receptors as an emerging chemical sensing scaffold, Biochemistry, 62, 187-195, 2022.
In this Perspective, we briefly overview recent advances in the biosensing of small molecules, including nucleic acid aptamers, allosteric transcription factors, and two-component systems. We then look more closely at a recently developed chemical sensing system, G protein-coupled receptor (GPCR)-based sensors. Finally, we consider the chemical sensing capabilities of the largest GPCR subfamily, olfactory receptors (ORs). We examine ORs’ role in nature, their potential as a biomedical target, and their ability to detect compounds not amenable for detection using other biological scaffolds. We conclude by evaluating the current challenges, opportunities, and future applications of GPCR- and OR-based sensors.

37. Marquez-Gomez P, Kruyer N, Eisen S, Torp L, Howie R, Jones E, France S, Peralta-Yahya P. Discovery of 8-hydroxyquinoline as a histamine receptor 2
blocker scaffold, ACS Synth Biol, 11, 2820-2828, 2022.
Histamine receptor 2 (HRH2) activation results in gastric acid secretion, and HRH2 blockers are used to treat peptidic ulcers and acid reflux. Some HRH2 blockers decomposes to N-nitrosodimethylamine, a human carcinogen, in the body. We developed a high-throughput HRH2-based sensor and use it to identify three novel HRH2 blockers, chlorquinaldol, chloroxine, and broxyquinoline. The blockers sharing an 8-hydroxyquinoline scaffold, which is not found among known HRH2 blockers. Taken together, this work demonstrates the utility of GPCR-based sensors for rapid drug discovery applications and identifies a novel HRH2 blocker scaffold.
blocker scaffold, ACS Synth Biol, 11, 2820-2828, 2022.
Histamine receptor 2 (HRH2) activation results in gastric acid secretion, and HRH2 blockers are used to treat peptidic ulcers and acid reflux. Some HRH2 blockers decomposes to N-nitrosodimethylamine, a human carcinogen, in the body. We developed a high-throughput HRH2-based sensor and use it to identify three novel HRH2 blockers, chlorquinaldol, chloroxine, and broxyquinoline. The blockers sharing an 8-hydroxyquinoline scaffold, which is not found among known HRH2 blockers. Taken together, this work demonstrates the utility of GPCR-based sensors for rapid drug discovery applications and identifies a novel HRH2 blocker scaffold.

2021
36. Chowdhury S. & Peralta-Yahya P. Two steps to sustainable polymers, Nat Chem, 13, 1157-1158, 2021.
35. Kruyer N., Realff M., Sun W., Genzale, C., Peralta-Yahya P. Designing the bioproduction of Martian rocket propellant via a biotechnology-enabled in
situ resource utilization strategy, Nat Commun, 12, 6166, 2021.
In the News: Gizmodo, Space.com, Popular Science
Mars colonization demands technological advances to enable the return of humans to Earth. Shipping the propellant and oxygen for a return journey is not viable. Considering the gravitational and atmospheric differences between Mars and Earth, we propose bioproduction of a Mars-specific rocket propellant, 2,3-butanediol (2,3-BDO), from CO2, sunlight and water on Mars via a biotechnology-enabled in situ resource utilization (bio-ISRU) strategy. Photosynthetic cyanobacteria convert Martian CO2 into sugars that are upgraded by engineered Escherichia coli into 2,3-BDO.
36. Chowdhury S. & Peralta-Yahya P. Two steps to sustainable polymers, Nat Chem, 13, 1157-1158, 2021.
35. Kruyer N., Realff M., Sun W., Genzale, C., Peralta-Yahya P. Designing the bioproduction of Martian rocket propellant via a biotechnology-enabled in
situ resource utilization strategy, Nat Commun, 12, 6166, 2021.
In the News: Gizmodo, Space.com, Popular Science
Mars colonization demands technological advances to enable the return of humans to Earth. Shipping the propellant and oxygen for a return journey is not viable. Considering the gravitational and atmospheric differences between Mars and Earth, we propose bioproduction of a Mars-specific rocket propellant, 2,3-butanediol (2,3-BDO), from CO2, sunlight and water on Mars via a biotechnology-enabled in situ resource utilization (bio-ISRU) strategy. Photosynthetic cyanobacteria convert Martian CO2 into sugars that are upgraded by engineered Escherichia coli into 2,3-BDO.

34. Kiattisewee C., Dong C., Fontana J., Sugianto W., Peralta-Yahya P., Carothers J., Zalatan, J. Portable bacterial CRISPR transcriptional activation enables
metabolic engineering in Pseudomonas putida, Metab Eng, 66, 283-295, 2021.
33. Yasi E. and Peralta-Yahya P. Screening for serotonin receptor 4 agonist using GPCR-based sensors in yeast, Methods in Molecular Biology, 2268,
77-84, 2021.
32. Kruyer, N., Sugianto W., Tickman B., Alba Burbano, D., Noireaux, V., Carothers, J., Peralta-Yahya P. Membrane augmented cell-free systems: a new
frontier in biotechnology, ACS Synth Biol, 10,670-681, 2021.
Cell-free systems have distinct advantages over living organisms in terms of toxicity tolerance and ease of downstream separations processes. However, conventional CFSs lack the ability to synthesize membrane proteins due to a lack of stabilizing interface. Here we review recent advances in membrane augmented CFSs, for the production of membrane proteins, with applications towards high-throughput enzyme screening, complex chemical biosynthesis, enzyme fuel cells, and targeted cell therapeutics using membrane-associated receptor proteins.
metabolic engineering in Pseudomonas putida, Metab Eng, 66, 283-295, 2021.
33. Yasi E. and Peralta-Yahya P. Screening for serotonin receptor 4 agonist using GPCR-based sensors in yeast, Methods in Molecular Biology, 2268,
77-84, 2021.
32. Kruyer, N., Sugianto W., Tickman B., Alba Burbano, D., Noireaux, V., Carothers, J., Peralta-Yahya P. Membrane augmented cell-free systems: a new
frontier in biotechnology, ACS Synth Biol, 10,670-681, 2021.
Cell-free systems have distinct advantages over living organisms in terms of toxicity tolerance and ease of downstream separations processes. However, conventional CFSs lack the ability to synthesize membrane proteins due to a lack of stabilizing interface. Here we review recent advances in membrane augmented CFSs, for the production of membrane proteins, with applications towards high-throughput enzyme screening, complex chemical biosynthesis, enzyme fuel cells, and targeted cell therapeutics using membrane-associated receptor proteins.

2020
31. Kruyer N., Wauldron N., Bommarius A., Peralta-Yahya P. Fully biological production of adipic acid analogs from branched catechols, Scientific
Reports, 10, 13367, 2020.
Bioproduction of adipic acid offers a renewable alternative to the chemical process. Starting from lignin-derived monomers provides is economic advantageous. However, lignin depolymerization results in a mixed monomer stream. Here we show that an enzyme cascade composed of catechol 1,2-dioxygenase and muconic acid reductase produces adipic acid and adipic acid analogs from catechol and alkyl substituted catechols likely formed during lignin depolymerization. This demonstrates the utility of this enzyme cascade for a variety of lignin-derived feedstocks and the ability to microbially produce adipic acid analogs that can be polymerized to form nylon-like polymers with enhanced properties.
30. Yasi E., Kruyer N., Peralta-Yahya P. Advances in high-throughput G-protein coupled receptor assays Curr Opin Biotechnol, 64, 210-217, 2020.
29. Kruyer N. and Peralta-Yahya P. Advancing the potential for the production of chemicals from carbon dioxide in Escherichia coli Biochemistry, 59,
731-732, 2020.
31. Kruyer N., Wauldron N., Bommarius A., Peralta-Yahya P. Fully biological production of adipic acid analogs from branched catechols, Scientific
Reports, 10, 13367, 2020.
Bioproduction of adipic acid offers a renewable alternative to the chemical process. Starting from lignin-derived monomers provides is economic advantageous. However, lignin depolymerization results in a mixed monomer stream. Here we show that an enzyme cascade composed of catechol 1,2-dioxygenase and muconic acid reductase produces adipic acid and adipic acid analogs from catechol and alkyl substituted catechols likely formed during lignin depolymerization. This demonstrates the utility of this enzyme cascade for a variety of lignin-derived feedstocks and the ability to microbially produce adipic acid analogs that can be polymerized to form nylon-like polymers with enhanced properties.
30. Yasi E., Kruyer N., Peralta-Yahya P. Advances in high-throughput G-protein coupled receptor assays Curr Opin Biotechnol, 64, 210-217, 2020.
29. Kruyer N. and Peralta-Yahya P. Advancing the potential for the production of chemicals from carbon dioxide in Escherichia coli Biochemistry, 59,
731-732, 2020.

2019
28. Yasi E., Allen A., Sugianto W., Peralta-Yahya P. Identification of three antimicrobials activating serotonin receptor 4 in colon cells ACS Synth Biol, 8,
2710-2717, 2019. In the news: GaTech
Agonists of the serotonin receptor 4 (5-HTR4) are used to treat irritable bowel syndrome with constipation. Today, there are no rapid assays for the identification of 5-HTR4 agonists. Here, we developed a 5-HTR4 assay capable of testing one compound per second and used it to screen >1,000 chemicals. We identified three antibiotic ligands that result in increased colon cell motility. This assay can be used to screen larger pharmaceutical libraries to identify novel treatments for IBS-C. This work shows that antimicrobials interact not only with the gut microbiota, but also with the human host.
28. Yasi E., Allen A., Sugianto W., Peralta-Yahya P. Identification of three antimicrobials activating serotonin receptor 4 in colon cells ACS Synth Biol, 8,
2710-2717, 2019. In the news: GaTech
Agonists of the serotonin receptor 4 (5-HTR4) are used to treat irritable bowel syndrome with constipation. Today, there are no rapid assays for the identification of 5-HTR4 agonists. Here, we developed a 5-HTR4 assay capable of testing one compound per second and used it to screen >1,000 chemicals. We identified three antibiotic ligands that result in increased colon cell motility. This assay can be used to screen larger pharmaceutical libraries to identify novel treatments for IBS-C. This work shows that antimicrobials interact not only with the gut microbiota, but also with the human host.

27. Yasi E., Eisen A., Wang H., Sugianto W., Minniefield A., Hoover K., Branham P., Peralta-Yahya P. Rapid deorphanization of human olfactory receptors in
yeast, Biochemistry, 58, 2160-2166, 2019.
Olfactory receptors are expressed ectopically outside the olfactory tissue. A key challenge in studying the role of ectopically expressed olfactory receptos (exORs) is the fact that most of them have no known compounds that activate them. We generated sensors for colon exORs and deorphanized 2 of them. We can now start understanding the role of olfactory receptors in the colon.
26. Aurand E*, Keasling J, Friedman D, Salis H, Liu C, Peralta-Yahya P, Carothers J, Arkin A, Collins A, Galm U, Cizauskas C, Haynes K, Lu A, Savage D,
Annaluru V, Bovenberg R, Carlson P, Contreras L, Freemont P, Hamazato F, Jewett M, Khalil A, Plassmeier J, Roubos H, Sampson J, Wook Chang M
Engineering biology: A research roadmap for the next-generation bioeconomy, Engineering Biology Research Consortium, 2019.
yeast, Biochemistry, 58, 2160-2166, 2019.
Olfactory receptors are expressed ectopically outside the olfactory tissue. A key challenge in studying the role of ectopically expressed olfactory receptos (exORs) is the fact that most of them have no known compounds that activate them. We generated sensors for colon exORs and deorphanized 2 of them. We can now start understanding the role of olfactory receptors in the colon.
26. Aurand E*, Keasling J, Friedman D, Salis H, Liu C, Peralta-Yahya P, Carothers J, Arkin A, Collins A, Galm U, Cizauskas C, Haynes K, Lu A, Savage D,
Annaluru V, Bovenberg R, Carlson P, Contreras L, Freemont P, Hamazato F, Jewett M, Khalil A, Plassmeier J, Roubos H, Sampson J, Wook Chang M
Engineering biology: A research roadmap for the next-generation bioeconomy, Engineering Biology Research Consortium, 2019.

2018
25. Sarria S., Bartholow T., Verga A., Burkart M., Peralta-Yahya P. Matching protein interfaces for improved medium-chain fatty acid production, ACS
Synth Biol, 7, 1179-1187, 2018.
Microbial production of chemicals relies on the introduction of enzymes from different organisms into a biotechnology accessible organism, such as Escherichia coli. Often, little attention is paid to the interaction between the heterologous and host enzymes. Here, we optimized the interphase of E. coli's acyl carrier protein (ACP) and Acinetobacter baylyi thioesterase to improve medium-chain fatty production.
25. Sarria S., Bartholow T., Verga A., Burkart M., Peralta-Yahya P. Matching protein interfaces for improved medium-chain fatty acid production, ACS
Synth Biol, 7, 1179-1187, 2018.
Microbial production of chemicals relies on the introduction of enzymes from different organisms into a biotechnology accessible organism, such as Escherichia coli. Often, little attention is paid to the interaction between the heterologous and host enzymes. Here, we optimized the interphase of E. coli's acyl carrier protein (ACP) and Acinetobacter baylyi thioesterase to improve medium-chain fatty production.

2017
24. Sarria S., Kruyer N., Peralta-Yahya P. Microbial synthesis of medium-chain chemicals form renewables, Nature Biotechnology, 35, 1158-1166, 2017.
23. Ehrenworth A., Claiborne T., Peralta-Yahya P. Medium-throughput screen of microbially produced serotonin via a GPCR-based sensor, Biochemistry,
56, 5471-5475, 2017.
A key limitation in the microbial production of chemicals is the throughput at which strains can be screened for chemical production. Chemical biosensors that convert chemical detection to a fluorescent output enable high-throughput metabolic engineering applications. Here, we engineer a yeast-based serotonin sensor, used it to detect microbially produced serotonin, and validated the assay for medium-throughput screening applications.
24. Sarria S., Kruyer N., Peralta-Yahya P. Microbial synthesis of medium-chain chemicals form renewables, Nature Biotechnology, 35, 1158-1166, 2017.
23. Ehrenworth A., Claiborne T., Peralta-Yahya P. Medium-throughput screen of microbially produced serotonin via a GPCR-based sensor, Biochemistry,
56, 5471-5475, 2017.
A key limitation in the microbial production of chemicals is the throughput at which strains can be screened for chemical production. Chemical biosensors that convert chemical detection to a fluorescent output enable high-throughput metabolic engineering applications. Here, we engineer a yeast-based serotonin sensor, used it to detect microbially produced serotonin, and validated the assay for medium-throughput screening applications.

22. Ehrenworth A., Haines M., Wong A., Peralta-Yahya P. Quantifying the efficiency of Saccharomyces cerevisiae translocation tags, Biotech Bioeng, 114,
2628-2636, 2017.
21. Kruyer N. and Peralta-Yahya P. Metabolic engineering strategies to bio-adipic acid production, Curr Opin Biotechnol, 45, 136-143, 2017.
20. Ehrenworth A., Peralta-Yahya P. Accelerating the semi-synthesis of alkaloid-based drugs through metabolic engineering. Nat Chem Biol, 13, 249-258,
2017.
In the news: GaTech COS, Phys.org, Health medicinet
Some pharmaceuticals are obtained via chemical derivatization of plant natural products. Today, plant pathways can be ported to microbes and by combining them with engineered enzymes, modified natural products that more closely resemble pharmaceuticals can be obtained. Here, we analyzed >2,000 FDA approved drugs and proposed pathways and engineered enzymes to produce modified plant alkaloids to accelerate the semi-synthesis of 7 pharmaceuticals currently on the market.
2628-2636, 2017.
21. Kruyer N. and Peralta-Yahya P. Metabolic engineering strategies to bio-adipic acid production, Curr Opin Biotechnol, 45, 136-143, 2017.
20. Ehrenworth A., Peralta-Yahya P. Accelerating the semi-synthesis of alkaloid-based drugs through metabolic engineering. Nat Chem Biol, 13, 249-258,
2017.
In the news: GaTech COS, Phys.org, Health medicinet
Some pharmaceuticals are obtained via chemical derivatization of plant natural products. Today, plant pathways can be ported to microbes and by combining them with engineered enzymes, modified natural products that more closely resemble pharmaceuticals can be obtained. Here, we analyzed >2,000 FDA approved drugs and proposed pathways and engineered enzymes to produce modified plant alkaloids to accelerate the semi-synthesis of 7 pharmaceuticals currently on the market.
2016
19. Amit I, Baker D, Barker R, Berger B, Bertozzi C, Bhatia S, Biffi A, Demichelis F, Doudna J, Dowdy SF, Endy D, Helmstaedter M, Junca H, June C, Kamb
S, Khvorova A, Kim DH, Kim JS, Krishnan Y, Lakadamyali M, Lappalainen T, Lewin S, Liao J, Loman N, Lundberg E, Lynd L, Martin C, Mellman I,
Miyawaki A, Mummery C, Nelson K, Paz J, Peralta-Yahya P, Picotti P, Polyak K, Prather K, Qin J, Quake S, Regev A, Rogers JA, Shetty R, Sommer M,
Stevens M, Stolovitzky G, Takahashi M, Tang F, Teichmann S, Torres-Padilla ME, Tripathi L, Vemula P, Verdine G, Vollmer F, Wang J, Ying JY, Zhang F,
Zhang T. Voices of biotech. Nat Biotechnol, 34, 270-275, 2016.
19. Amit I, Baker D, Barker R, Berger B, Bertozzi C, Bhatia S, Biffi A, Demichelis F, Doudna J, Dowdy SF, Endy D, Helmstaedter M, Junca H, June C, Kamb
S, Khvorova A, Kim DH, Kim JS, Krishnan Y, Lakadamyali M, Lappalainen T, Lewin S, Liao J, Loman N, Lundberg E, Lynd L, Martin C, Mellman I,
Miyawaki A, Mummery C, Nelson K, Paz J, Peralta-Yahya P, Picotti P, Polyak K, Prather K, Qin J, Quake S, Regev A, Rogers JA, Shetty R, Sommer M,
Stevens M, Stolovitzky G, Takahashi M, Tang F, Teichmann S, Torres-Padilla ME, Tripathi L, Vemula P, Verdine G, Vollmer F, Wang J, Ying JY, Zhang F,
Zhang T. Voices of biotech. Nat Biotechnol, 34, 270-275, 2016.

2015
18. Ehrenworth A., Sarria S., Peralta-Yahya P. Pterin-dependent mono-oxidation for the microbial synthesis of a modified monoterpene indole alkaloid
ACS Synth Biol, 4, 1295-1307, 2015.
In the news: Petit Institute News
Anticancer and antimalarial agents are found among plant alkaloids. Isolation of alkaloids from plants is challenging due to their low accumulation and difficult separation from other similar compounds. To become pharmaceuticals, alkaloids often need to be derivatized to increase bioavailability and reduce toxicity. Here, we engineered the first microbial platform for the production of a modified monoterpene indole alkaloid from simple sugars.
18. Ehrenworth A., Sarria S., Peralta-Yahya P. Pterin-dependent mono-oxidation for the microbial synthesis of a modified monoterpene indole alkaloid
ACS Synth Biol, 4, 1295-1307, 2015.
In the news: Petit Institute News
Anticancer and antimalarial agents are found among plant alkaloids. Isolation of alkaloids from plants is challenging due to their low accumulation and difficult separation from other similar compounds. To become pharmaceuticals, alkaloids often need to be derivatized to increase bioavailability and reduce toxicity. Here, we engineered the first microbial platform for the production of a modified monoterpene indole alkaloid from simple sugars.

17. Ort DR, Merchant SS, Alric, Blankenship RE, Bock R, Croce R, Hanson MR, Hibberd JM, Long SP, Moore TA, Moroney J, Niyogi KK, Parry MA, Peralta-
Yahya P., Prince RC, Redding KE, Spalding MH, van Wijk KJ, Vermaas WF, von Caemmerer S, Weber AP, Yeates TO, Yuan JS, Zhu XG. Redesigning
photosynthesis to sustainably meet global food and bioenergy demand, Proc. Natl Acad Sci USA, 112, 8529-36, 2015.
In the news: Gizmodo
16. Mukherjee K., Bhattacharyya S., Peralta-Yahya, P. GPCR-based chemical sensors for medium-chain fatty acids, ACS Synth Biol, 4, 1261-9, 2015.
In the news: Petit Institute News
Chemical biosensors convert chemical detection to a fluorescent or colorimetric output, enabling the screening of microbially produced chemicals up to 5 orders of magnitude faster than mass spectrometry approaches. Here, we developed a platform for the generation of G-protein coupled receptor (GPCR)-based sensors in yeast.
Yahya P., Prince RC, Redding KE, Spalding MH, van Wijk KJ, Vermaas WF, von Caemmerer S, Weber AP, Yeates TO, Yuan JS, Zhu XG. Redesigning
photosynthesis to sustainably meet global food and bioenergy demand, Proc. Natl Acad Sci USA, 112, 8529-36, 2015.
In the news: Gizmodo
16. Mukherjee K., Bhattacharyya S., Peralta-Yahya, P. GPCR-based chemical sensors for medium-chain fatty acids, ACS Synth Biol, 4, 1261-9, 2015.
In the news: Petit Institute News
Chemical biosensors convert chemical detection to a fluorescent or colorimetric output, enabling the screening of microbially produced chemicals up to 5 orders of magnitude faster than mass spectrometry approaches. Here, we developed a platform for the generation of G-protein coupled receptor (GPCR)-based sensors in yeast.
15. Voices of chemical biology. Nat Chem Biol. 11, 378-179, 2015.
14. Peralta-Yahya P. Biosensor keep DOPA on track, News & Views, Nat Chem Biol, 11, 450-1, 2015.
In the news: PBS NewsHour, Science Magazine, Science News
2014
13. Sarria S., Wong B., Garía Martín H., Keasling J., Peralta-Yahya P. Microbial synthesis of pinene, ACS Synth Biol, 3, 466-75, 2014.
In the news: Science Daily, Popular Science, Scientific American, Chemical and Engineering News
Postdoctoral and graduate work
12. de Rond T., Peralta-Yahya P., Cheng X., Northen T.R., Keasling J.D. Versatile synthesis of probes for high-throughput enzyme activity screening, Anal
Bioanal Chem, 405, 4969-73, 2013.
11. Romanini D., Peralta-Yahya P., Mondol V., Cornish V.W. A heritable recombination system for synthetic darwinian evolution in yeast, ACS Synth Biol, 1,
602-9, 2012.
10. Peralta-Yahya P., Zhang F., del Cardayre S.B., Keasling J.D. Microbial engineering for the production of advanced biofuels, Nature, 488, 320-28, 2012.
9. McAndrew R,P., Peralta-Yahya P., DeGiovanni A., Pereira J.H., Hadi M.Z., Keasling J.D., Adams P.D. Structure of a three-domain sesquiterpene synthase:
a prospective target for advanced biofuels production. Structure, 19, 1875-84, 2011.
8. Peralta-Yahya P., Ouellet M., Chan R., Mukhopadhyay A., Keasling J.D., Lee T.S. Identification and microbial production of a terpene-based advanced
biofuel, Nat Commun 2:483, doi: 10.1038/ncomms1494, 2011.
7. Bokinsky G., Peralta-Yahya P., George A., Holmes B.M., Steen E.J., Dietrich J., Lee T.S., Tullman-Ercek D., Voigt C.A., Simmons B.A., Keasling J.D
Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli, Proc Natl Acad Sci USA. 108,
19949-54, 2011.
6. Pirakitikulr N., Ostrov N., Peralta-Yahya P., Cornish, V.W. PCRless library mutagenesis via oligonucleotide recombination in yeast. Protein Sci, 19,
2336-46, 2010.
5. Peralta-Yahya P., Keasling J.D. Advanced biofuel production in microbes, Biotechnol J, 5, 147-62, 2010.
4. Peralta-Yahya P., Carter B.T., Lin H., Tao H., Cornish V.W. High-throughput selection for cellulase catalysts using chemical complementation, J Am
Chem Soc, 130, 17446-52, 2008.
3. Tao H., Peralta-Yahya P., Decatur J., Cornish V.W. Characterization of a new glycosynthase cloned by using chemical complementation,
ChemBioChem,9, 681-4, 2008.
2. Peralta-Yahya P., Cornish V.W. Bringing the power of genetics to chemistry, in Chemical Biology, S.L. Schreiber, T. Kapoor, G. WEiss, Eds. Wiley-VCH
Verlag, 2007.
1. Tao H., Peralta-Yahya P., Lin H., Cornish V.W., Optimized design and synthesis of chemical dimerizer substrates for detection of glycosynthase activity
via chemical complementation, Bioorg Med Chem, 14, 6940-53, 2006.
14. Peralta-Yahya P. Biosensor keep DOPA on track, News & Views, Nat Chem Biol, 11, 450-1, 2015.
In the news: PBS NewsHour, Science Magazine, Science News
2014
13. Sarria S., Wong B., Garía Martín H., Keasling J., Peralta-Yahya P. Microbial synthesis of pinene, ACS Synth Biol, 3, 466-75, 2014.
In the news: Science Daily, Popular Science, Scientific American, Chemical and Engineering News
Postdoctoral and graduate work
12. de Rond T., Peralta-Yahya P., Cheng X., Northen T.R., Keasling J.D. Versatile synthesis of probes for high-throughput enzyme activity screening, Anal
Bioanal Chem, 405, 4969-73, 2013.
11. Romanini D., Peralta-Yahya P., Mondol V., Cornish V.W. A heritable recombination system for synthetic darwinian evolution in yeast, ACS Synth Biol, 1,
602-9, 2012.
10. Peralta-Yahya P., Zhang F., del Cardayre S.B., Keasling J.D. Microbial engineering for the production of advanced biofuels, Nature, 488, 320-28, 2012.
9. McAndrew R,P., Peralta-Yahya P., DeGiovanni A., Pereira J.H., Hadi M.Z., Keasling J.D., Adams P.D. Structure of a three-domain sesquiterpene synthase:
a prospective target for advanced biofuels production. Structure, 19, 1875-84, 2011.
8. Peralta-Yahya P., Ouellet M., Chan R., Mukhopadhyay A., Keasling J.D., Lee T.S. Identification and microbial production of a terpene-based advanced
biofuel, Nat Commun 2:483, doi: 10.1038/ncomms1494, 2011.
7. Bokinsky G., Peralta-Yahya P., George A., Holmes B.M., Steen E.J., Dietrich J., Lee T.S., Tullman-Ercek D., Voigt C.A., Simmons B.A., Keasling J.D
Synthesis of three advanced biofuels from ionic liquid-pretreated switchgrass using engineered Escherichia coli, Proc Natl Acad Sci USA. 108,
19949-54, 2011.
6. Pirakitikulr N., Ostrov N., Peralta-Yahya P., Cornish, V.W. PCRless library mutagenesis via oligonucleotide recombination in yeast. Protein Sci, 19,
2336-46, 2010.
5. Peralta-Yahya P., Keasling J.D. Advanced biofuel production in microbes, Biotechnol J, 5, 147-62, 2010.
4. Peralta-Yahya P., Carter B.T., Lin H., Tao H., Cornish V.W. High-throughput selection for cellulase catalysts using chemical complementation, J Am
Chem Soc, 130, 17446-52, 2008.
3. Tao H., Peralta-Yahya P., Decatur J., Cornish V.W. Characterization of a new glycosynthase cloned by using chemical complementation,
ChemBioChem,9, 681-4, 2008.
2. Peralta-Yahya P., Cornish V.W. Bringing the power of genetics to chemistry, in Chemical Biology, S.L. Schreiber, T. Kapoor, G. WEiss, Eds. Wiley-VCH
Verlag, 2007.
1. Tao H., Peralta-Yahya P., Lin H., Cornish V.W., Optimized design and synthesis of chemical dimerizer substrates for detection of glycosynthase activity
via chemical complementation, Bioorg Med Chem, 14, 6940-53, 2006.